Rosenior was sent-off in the 2-0 defeat at Swansea 10 days ago and will miss out through a one-match suspension.
With Graeme Murty on loan at Charlton manager Steve Coppell will have to hand youngster Julian Kelly his first league start against the Championship leaders.
Defender Andre Bikey is back from his own one-match ban but has sustained ankle ligament damage which will keep him out.
Adam Federici will continue in goal but first-choice Marcus Hahnemann has returned to training after being out for six weeks with a calf injury.
The Royals have had 10 days off since their defeat at Swansea and a win over Mick McCarthy's side could lift them to within two points of Wolves.
Wolves manager Mick McCarthy is expected to make wholesale changes to his starting line-up for the tricky trip.
New loan signing Nigel Quashie was ineligible for Saturday's FA Cup clash with Middlesbrough and comes into contention for his debut, while Richard Stearman is available after serving a one-game suspension.
Key attacking trio Michael Kightly, Sylvan Ebanks-Blake and Andy Keogh started the Boro game on the bench and all three should be restored to the starting XI.
Stephen Ward, who has missed Wanderers' last two games with a shoulder problem, sustained a nasty gash to his shin in training on Sunday which required stitches, but McCarthy is confident he will be fit.
Chris Iwelumo is also expected to recover from an ankle injury to be involved, but goalkeeper Carl Ikeme remains sidelined with a back problem.