Glen Little, Michael Duberry, Ben Hamer and Scott Davies are all out of contract this summer but have been offered terms to stay.
However, John Oster, Ulises De La Cruz, John Halls, Graham Stack, Adam Bygrave and Aaron Brown will all leave the Madejski as their contracts have come to an end.
"I would like to place on record my thanks to all of the players who will be moving on," director of football Nick Hammond told the club's website, www.readingfc.co.uk.
"They have all shown great professionalism, and we wish them all the very best for the future."